Principal cast[]
- Diedrich Bader – Batman, Kilowog, Ace, Owlman, Solomon Grundy, Punch, Gorilla Boss, Adult Damian Wayne/Future Batman, Lord Death Man, Creepy Usher, Cave Batman, Pirate Batman, Batmanicus, Robot Batman, Musketeer
- Corey Burton – Red Tornado, Silver Cyclone, Thomas Wayne (in "Invasion of the Secret Santas"), False-Face, Doctor Mid-Nite, Doc Magnus, Mercury, Chancellor Gor-Zonn, General Zahl, Killer Moth, Batman (Bat-Manga), Joker (Scooby-Doo version), Alan Scott
- John DiMaggio – Aquaman, Gorilla Grodd, Tiger Men, Typhon, Enemy Ace, Ubu, Faceless Hunter, Black Adam, Black Mask, Tattoo, Pharaoh, Toyman, Vigilante, Hellgrammite, Mr. Freeze, Legionary, Captain Boomerang, Owen
- Will Friedle – Blue Beetle (Jaime Reyes), Scarlet Scarab, Lazy Eye
- Tom Kenny – Plastic Man, Baby Face, The Ray, Deadshot, Mirror Master, 'Mazing Man
- James Arnold Taylor – Green Arrow, Blue Bowman, Guy Gardner, Major Disaster, Wotan, Nabu, Mark Desmond, Arges, Rocky Davis, Alpha-Red, G.I. Robot, Jace
Special guest cast[]
- Morena Baccarin – Cheetah
- Edoardo Ballerini – Vulture, Jack
- Xander Berkeley – Sinestro
- Clancy Brown – Per Degaton, Rohtul
- Gabrielle Carteris – Vicki Vale, Princess Laethwen
- Patrick Cavanaugh – Damian Wayne
- Mindy Cohn – Velma Dinkley
- Jeffrey Combs – Kite Man
- Kevin Conroy – Batman of Zur-En-Arrh, Phantom Stranger
- Tim Conway – Weeper
- Olivia d'Abo – Elasti-Girl
- Diane Delano – Big Barda
- Dana Delany – Vilsi Valar
- Michael Dorn – Bane, Kru'll the Eternal
- Greg Ellis – Gentleman Ghost, Doctor Fate, Cavalier, Dr. Canus, Hawk, Thomas Wayne (in "Dawn of the Deadman!"), Shrapnel, Mr. Mind, Batman of 800,000 A.D.
- R. Lee Ermey – Wildcat
- Oded Fehr – Equinox
- Ellen Greene – Mrs. Manface
- Ioan Gruffudd – Blue Beetle Scarab, Red Ryan
- Mark Hamill – The Spectre
- Neil Patrick Harris – Music Meister
- Tippi Hedren – Hippolyta
- John Michael Higgins – Riddler
- William Katt – Hawkman (Carter Hall)
- Wallace Langham – Ocean Master
- Loren Lester – Hal Jordan
- Vicki Lewis – Wonder Woman, Star Sapphire
- Matthew Lillard – Shaggy Rogers
- Carl Lumbly – Tornado Champion/Tornado Tyrant
- Tim Matheson – Jarvis Kord
- David McCallum – Merlin
- Ted McGinley – Aquaman
- Andy Milder – Flash (Jay Garrick)
- Richard Moll – Lew Moxon, Two-Face (substitute voice)
- Phil Morris – Fox, Jonah Hex
- Laraine Newman – Ms. Minerva
- Julie Newmar – Martha Wayne (2nd voice)
- Gary Owens – Space Ghost
- Hunter Parrish – Kid Flash, Geo-Force
- Ron Perlman – Doctor Double X
- Jim Piddock – Calendar Man/Calendar King, Doctor Watson, Doctor Sivana, Shazam, Thaddeus Sivana, Jr.
- James Remar – Two-Face
- Paul Reubens – Bat-Mite
- Henry Rollins – Robotman
- Jeffrey Ross – Himself
- Michael Rosenbaum – Deadman
- Stephen Root – Penguin, Woozy Winks, Planet Master, Killer Croc
- Peter Scolari – Atom (Ray Palmer)
- Tom Everett Scott – Booster Gold
- Armin Shimerman – Calculator, Psycho-Pirate, Prof Haley, Guardians of the Universe
- John Wesley Shipp – Professor Zoom
- J.K. Simmons – Guardians of the Universe, Evil Star, Ace Morgan
- Cree Summer – Vixen
- Jeffrey Tambor – Crazy Quilt
- Tony Todd – Astaroth
- Alan Tudyk – Flash (Barry Allen)
- Michael T. Weiss – Adam Strange
- Adam West – Thomas Wayne (3rd voice), Proto Bat-Bot
- Wil Wheaton – Blue Beetle (Ted Kord)
- Michael Jai White – Tattooed Man
- Gary Anthony Williams – Fun Haus, Mongul, Mongal
- Tyler James Williams – Firestorm
- Wade Williams – Mantis, Supreme Chairman of Qward
- Thomas F. Wilson – Sportsmaster, Catman
- Henry Winkler – Ambush Bug
- Peter Woodward – Caesar, Ra's al Ghul
- Michael-Leon Wooley – Kalibak, Darkseid
- "Weird Al" Yankovic – Himself, Mr. Star
Additional voices[]
- Sebastian Bader – Damian Wayne's son/Future Robin
- Dee Bradley Baker – Clock King, Etrigan, Felix Faust, Brain, Chemo,Scarecrow, Ace the Bathound, Oberon, General Ramjam, Dove, Brother Eye, Global Peace Agency Operative, Fisherman, Professor Zee, Tin, Professor Milo, Animal-Vegetable-Mineral Man, Starro, Haunted Tank, Madniks, Bug-Eyed Bandit, Mister Atom, Misfit, Warren Griffith, Vincent Velcro, Pvt. Elliot "Lucky" Taylor, John Wilkes Booth, Punchichi
- Jeff Bennett – Joker, Red Hood, OMAC, Captain Marvel, Batman's Singing Voice, Rubberneck, Abra Kadabra, Joker-Mite, Joker Jr., Rubin, Penguin (Scooby-Doo version), Prez, El Gaucho, Ultra-Humanite, Starman
- Gregg Berger – Hammertoes, The Brain, Police Captain, Crime Boss, Creature King
- Brian Bloom – Iron, Oxygen, Creeper, Rip Hunter, Captain Atom
- Steven Blum – Heat Wave, Captain Cold
- Andrea Bowen – Talia al Ghul
- Ian Buchanan – Sherlock Holmes
- Cathy Cavadini – Alanna, Jan, Ruby Ryder, Fiona, Dr. Myrra Rhodes
- Grey DeLisle – Fire, Black Canary, Daphne Blake, Robin (Bat-Manga), Dala
- John DeVito – Captain Marvel, Jr.
- Sean Donnellan – Elongated Man, Steve Trevor
- Robin Atkin Downes – Weather Wizard, Kobra, Firefly, Ten-Eyed Man
- Bill Fagerbakke – Ronnie Raymond, Lead, Helium, Riddler Henchman
- Nika Futterman – Lashina, Catwoman
- James Garrett – Alfred Pennyworth
- Zachary Gordon – Young Bruce Wayne, Young Aqualad
- Richard Green – General Kreegaar
- Kim Mai Guest – Katana (2nd voice)
- Nicholas Guest – Question, Martian Manhunter
- Jennifer Hale – Ramona O'Brian, Poison Ivy (1st voice), Zatanna, Killer Frost, Ice
- David K. Hill – Negative Man
- Sirena Irwin – Mera, Lois Lane
- Lauri Johnson – Ma Murder
- Mikey Kelley – Kamandi
- Lex Lang – Doctor Polaris, Hourman, Gold, Hydrogen, Alloy, Young Wildcat, Adult Dick Grayson
- Hope Levy – Stargirl, Phantom Lady
- Yuri Lowenthal – Mister Miracle, Prince Tuftan, Bulletman
- Tress MacNeille – Ms. Gatsby
- Jason Marsden – Paco, Speedy, Robin (Scooby-Doo)
- Vanessa Marshall – Poison Ivy (2nd voice), Batwoman
- Richard McGonagle – Sardath, Professor Carter Nichols, The Chief, Perry White, Brainiac
- Scott Menville – Metamorpho
- Jason C. Miller – Doll Man, Black Condor
- Pat Musick – Martha Wayne ("Dawn of the Deadman!")
- Ryan Ochoa – Young Speedy
- Peter Onorati – Joe Chill
- Vyvan Pham – Katana (1st voice)
- Alexander Polinsky – Slug, G'nort, Jimmy Olsen
- Rachel Quaintance – Carol Ferris
- Enn Reitel – Chancellor Deraegis
- Peter Renaday – Uncle Sam, Abraham Lincoln, Cartoon Network Announcer
- Kevin Michael Richardson – Black Manta, B'Wana Beast , Despero, Steppenwolf, Blockbuster, Detective Chimp, Monsieur Mallah, Starro, Telle-Teg, President Barack Obama, Lex Luthor, Mister Mxyzptlk, Heroes Voiceover
- Bumper Robinson – Black Lightning
- Roger Rose – Tom Tyler, Superman, Amazo
- Eliza Schneider – Baroness Von Gunther, Georgette Taylor
- Jeremy Shada – Young Robin
- Zack Shada – Aqualad
- James Sie – Atom (Ryan Choi), Dyna-Mite
- Jane Singer – Jewelee
- Meghan Strange – Harley Quinn
- Preston Strother – Arthur Curry Jr., Kyle
- Tara Strong – Huntress, Billy Batson, Mary Marvel, Georgia Sivana
- Gary Sturgis – Bronze Tiger
- Fred Tatasciore – Mutant Master, Arsenal, Sgt. Rock, Major Force
- Hynden Walch – Carbon Dioxide, Platinum
- Frank Welker – Scooby-Doo, Fred Jones, Bat Boy, Batman (Scooby-Doo version)
- Billy West – Skeets
- Mae Whitman – Batgirl
- Tyrel Jackson Williams – Chris
- Crawford Wilson – Robin
- Marc Worden – Kanjar Ro, Lt. Matthew Shrieve
- Tatyana Yassukovich – Morgaine Le Fay
- Keone Young – Global Peace Agency Operative
Crew[]